阅读方式:

Immunomodulatory effects of antifungal agents on the response of human monocytic cells to Aspergillus fumigatus conidia.

出处: Med Mycol   2010   Aug   48 (5) :704-9

作者:Choi JH;Kwon EY;Park CM;Choi SM;Lee DG;Yoo JH;Shin WS;Stevens DA

PMID:20156030

We evaluated the immunomodulatory effects of three different classes of antifungal agents on the human monocytic cell line, THP-1, which had been stimulated in vitro with Aspergillus fumigatus conidia. Cells treated with amphotericin B (AmB), micafungin (MF), and voriconazole (VCZ), at concentrations not affecting cell viability, reduced production of tumor necrosis factor (TNF)-alpha in response to conidia, with the greatest reduction noted with VCZ. The reduction of TNF-alpha production correlated with TNF-alpha gene expression assessed by PCR and nuclear factor kappaB (NF kappaB) levels. Co-stimulation with granulocyte-macrophage colony stimulating factor abolished immunomodulatory effects of the drugs. Antifungal agents affect the immune reaction caused by A. fumigatus conidia in stimulated monocytes at clinically relevant drug concentrations. Because drugs with different mechanisms of action produced this effect, this suggests that it is the result of factors mediated by the cells. The impact of these immunomodulatory effects needs assessment.